Fig. 4 | Scientific Reports

Fig. 4

From: CD38 modulates cytokine secretion by NK cells through the Sirt1/NF-κB pathway, suppressing immune surveillance in colorectal cancer

Fig. 4

The effect of CD38 on the expression of Sirt1, NF-κB and acetyl-NF-κB in NK cells. (A) Gene expression levels of Sirt1, CD38, and NF-κB in CD38 + NK cells were determined via real-time PCR. (B) Protein expression levels of CD38, Sirt1, NF-κB, and acetyl-NF-κB were measured via Western blot analysis. Quantitation of the protein expression was performed for CD38 (C) Sirt1 (D), NF-κB (E), and acetyl-NF-κB (F) in CD38 + NK cells from different groups. GAPDH was used as an internal control. CD38 + NK (HC): CD38 + NK cells isolated from the blood of HCs; CD38 + NK (CRC): CD38 + NK cells isolated from the blood of CRC patients; HCs: healthy controls; CRC: colorectal cancer. CD38 mAb: anti-CD38 monoclonal antibody; C-IgG: control IgG. **: P < 0.01 and ***: P < 0.001.

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