Fig. 1 | Scientific Reports

Fig. 1

From: Inserting Omp22 into the flagellin protein, replacing its hypervariable region, results in stronger protection against lethal Acinetobacter baumannii infection

Fig. 1

Design and evaluation of fusion FliC-Omp22 protein. (A) Diagram illustrating the design and construction of FliC-Omp22. The D3 domain of FliC protein (residues 185 to 285) was removed and substituted with the Omp22 protein. (B) Schematic representation of the linear B-cell epitopes identified by BepiPred-1.0, BepiPred-2.0, ABCpred, LBtope, BcePred and BCPred tools. (C) Robetta’s prediction for the 3D structure of FliC-Omp22. (D) Western blot analysis shows purified recombinant Omp22, FliC, and FliC-Omp22 proteins in lanes 1, 2, and 3, respectively, identified using an anti-His tag antibody.

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