Fig. 2 | Scientific Reports

Fig. 2

From: Inserting Omp22 into the flagellin protein, replacing its hypervariable region, results in stronger protection against lethal Acinetobacter baumannii infection

Fig. 2

Antigenicity, integrity and bioactivity of FliC-Omp22 fusion protein. (A) In the protein ELISA assay, recombinant FliC-Omp22 showed binding to anti-Omp22 and anti-FliC specific antibodies, compared to normal mouse serum, which showed no binding. (B) In the immunoblot analysis, anti-FliC antibody specifically reacted with FliC (lane 2, ∼51 kDa) and FliC-Omp22 (lane 3, ∼64 kDa) proteins and did not show reactivity with Omp22 (lane 1). (C) Anti-Omp22 antibody specifically bound to Omp22 (lane 1, ∼25 kDa) and FliC-Omp22 (lane 2, ∼64 kDa) proteins and did not show reactivity with FliC proteins (lane 3). (D) Anti-FliC-Omp22 antibody exhibited reactivity with FliC (lane 1, ∼51 kDa), Omp22 (lane 2, ∼25 kDa), and FliC-Omp22 (lane 3, ∼64 kDa) proteins. Lane M denotes the positions of molecular weight markers. (E) FliC-Omp22, similar to FliC, induced TNF-α production in TLR5-positive RAW 264.7 cells in a concentration-dependent manner. The data are represented as the mean ± standard deviation (SD).

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