Fig. 7
Multiplex RNA profiling of Mtb-infected macrophages treated with PBA or selected sirtuin inhibitors. Uninfected (white bars) and H37Rv-infected hMDMs (MOI5) (grey bars) were treated with medium only (Control or MOI5), PBA, tenovin (Ten), suramin (Sur), salermide (Sal) or cambinol (Cam) and assessed for mRNA expression of (A) IL-1β, (B) IL-6, (C) TNFα, (D) IL-8 (CXCL8), (E) CCL4, (F) CCL5, (G) SOCS-3, (H) PTGS-2 (COX2), (I) IL-10, (J) NFκB, (K) CAMP (LL-37), and (L) TLR2. Data assessed with Taqman array cards at 24 h is presented in box and whiskers graphs (median and min/max) from n = 5 donors (n = 3–4 donors for uninfected groups) and was analyzed using a Friedman and Dunn´s multiple comparisons test (Mtb-infected groups only). mRNA fold change in uninfected and untreated cells was set to 1. mRNA levels that are not visible in uninfected cells (A, B, D and H) due to a large fold induction in matched Mtb-infected cells, were in the range of 0-10-fold as compared to untreated and uninfected cells. *P < 0.05, **P < 0.01.
