Fig. 6
Cutting the optic nerve with a laser has a slight effect on gene expression in the retina at 3 days PL. (A) Retinas from experimental and control eyes were collected for RNA isolation three days after optic nerve transection. (B) RNA-seq data indicate that counts per million (CPM) distributions for control (Ret_C) and experimental (Ret_L) retinas do not differ significantly 3 days after injury. (C) To demonstrate the correlation (Corr) between gene expression in Ret_C vs. Ret_L retinas, scatter plots were utilized (D) The principal component analysis (PCA) shows a slight difference between control and experimental retinas. (E) The heatmap shows genes whose expression differs greatly in Ret_C vs. Ret_L retinas. (F) The volcano plot shows that the number of genes whose expression was statistically significantly (FDR < 0.1) decreased or increased |lfc| >1 in experimental and control retinas was small. (G) Subtle changes in gene expression are reflected in the low representation of significant biological processes or cellular components that may play a role in retinas with injured optic nerves.
