Fig. 5
From: Development of an efficient NUPR1 inhibitor with anticancer activity
AJO14 treatment induced mitochondrial metabolic failure. (A) Total ATP content measured in MIA PaCa-2 cells incubated with AJO14 for 24 h. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (2-way ANOVA, Dunnett’s test) (n = 3). (B and C) Oxygen consumption rate measured in MIA PaCa-2 cells using a Seahorse XF bioanalyzer after 24 h of treatment with increasing concentrations of (B) AJO14, and in the presence of AJO14 (10 μM) with increasing concentrations of (C) Olaparib. A representative experiment is shown (n = 3).
