Fig. 1

Characterization of the Cre-reporter transgenic mouse model with membrane-targeted tdTomato (mT). (A) Mating strategy to produce mT+—expressing progeny. A two-color fluorescent Cre-reporter allele (mT/mG construct), which has a membrane-targeted tandem dimer Tomato (mT) red fluorescent protein is expressed in all cells and tissues of the progeny. (B) PCR revealed the presence of the mouse SRY (M-SRY) gene in the DNA of the cell population extracted from the mT+ cells of different tissues. The positive control DNA was isolated from lung cells of the male TdTomato, and the negative control DNA was isolated from lung cells of the C57 wild-type mice. The PCR product of M-SRY sample was run in the 2% gel. (C) Maternal lung tissue sections were imaged under the fluorescence microscope for the presence of mT+ cells (red fluorescence indicated by yellow arrows). We observed an increased in red color intensity (TdTomato) of E. coli-treated mice tissues compared to term-delivered mice. This is the representative fluorescence image of the remaining tissues. Flow data represented as the frequency of cell population (mean values) (Fig. 2). Fetal-specific immune cell characterization in maternal lung tissues at different pregnant conditions.