Fig. 10
From: Delayed vacuolation in mammalian cells caused by hypotonicity and ion loss

3T3 cells were exposed to 33% DMEM with or without bafilomycin A1 and observed under phase contrast at specified times. The numbers of visible vacuoles with and without bafilomycin A1 were as follows: 23 and 3 (0.5 h), 118 and 74 (2 h), and 227 and 77 (6 h), respectively. Scale bar, 25 μm.