Fig. 3
From: Delayed vacuolation in mammalian cells caused by hypotonicity and ion loss

Phase contrast images of the LateVacs. (A) 3T3 cells incubated in the gluconate buffer. (B) 3T3 cells incubated in the low-sodium buffer. (C) HeLa cells incubated in the nitrate buffer. All the incubations were carried out for 6 h. These images cannot necessarily be seen as representative, because the size and the number of vacuoles varied between experiments (see Table 1), but they were common enough. Scale bar, 20 μm.