Fig. 4
From: Delayed vacuolation in mammalian cells caused by hypotonicity and ion loss

The effect of double WNK1/WNK3 knockout (DKO) on vacuolation. Wildtype and mutant HEK cells were observed after a 6 h incubation in the hypotonic solution (33% DMEM) or in a low-chloride buffer based on sodium nitrate. Also see Table 2 for quantification. Scale bar, 25 µm.