Fig. 5
From: Delayed vacuolation in mammalian cells caused by hypotonicity and ion loss
Confocal images of lipid staining reveal the membrane surrounding a single hypotonically-induced LateVac in HeLa (incubated overnight in 25% DMEM). The contrast inversion in bright field at defocusing indicates that the vacuoles have a low dry mass concentration: while optically denser compartments show a dark rim at overfocusing (the objective positioned too far from the object, BF1) and a light rim at underfocusing (objective brought too close, BF2), the vacuoles display the opposite behavior. Scale bar, 10 μm.
