Fig. 7
From: Delayed vacuolation in mammalian cells caused by hypotonicity and ion loss

Negative correlation between phase contrast intensity and LysoTracker fluorescence. Red squares mark the vesicles with low protein density (i.e., vacuoles) and no fluorescence; blue squares mark the areas with high protein density and LysoTracker staining. Both phase contrast and fluorescence images of 3T3 cells were collected by the same 40 × phase contrast objective. Scale bar, 5 μm.