Table 3 Comparison of the features of hypotonic vacuoles in different studies.
From: Delayed vacuolation in mammalian cells caused by hypotonicity and ion loss
Feature | Iwasa et al.32 | Li et al.33 | This work and Hollembeak and Model28 |
|---|---|---|---|
Cell types | 3T3-L1 | Cos, HeLa, Hap | 3T3, HeLa, chondrocytes |
Timing |  >  > 30 min | 30 min |  >  > 30 min |
Reversibility | Yes | Â | Yes |
Cl- depletion | Inhibits | Â | Stimulates |
Cl- channel blocker | Inhibits | Inhibits | Inhibits |
Hg2+ | No effect | Inhibits | No effect* |
Bafilomycin A1 | Â | Inhibits | No effect |
Cytochalasin | Â | Â | Stimulates |
LAMP-1 |  |  +  |  +  |
Acridine orange |  −  |  |  −  |
LysoTracker |  |  +  |  −  |
Calcein AM |  |  +  |  −  |
Dextran |  + / −  |  +  |  −  |
