Fig. 2

Spontaneous non-social olfactory habituation/dishabituation and discrimination task in WT and Fmr1 KO mice. (a) Absolute (s) and relative (%) total sniffing duration during the non-social olfactory habituation/dishabituation test in WT and Fmr1 KO males (WT: n = 16, Fmr1 KO: n = 17). (b) Discrimination between two non-social odors (banana and vanilla) in WT and Fmr1 KO males. (c) Absolute (s) and relative (%) total sniffing duration during the non-social olfactory habituation/dishabituation test in WT and Fmr1 KO females (WT: n = 17, Fmr1 KO: n = 15). (d) Discrimination between two non-social odors (banana and vanilla) in WT and Fmr1 KO females. For the non-social olfactory habituation/dishabituation test (a, c) three odorant-paired sessions were used in following order: 1) water, 2) banana, and 3) vanilla. Each session consists of three consecutive trials (T1–T3) with 2 min inter-session interval. The statistical significances of the habituation (T1 vs. T3) and dishabituation (T3 vs. T1 of following session) for each genotype were evaluated using permutational paired t-test, \(^{\blacktriangle}\)P < 0.05, \(^{\blacktriangle \blacktriangle}\)P < 0.01, \(^{\blacktriangle \blacktriangle \blacktriangle}\)P < 0.001 for habituation, and ^◆◆P < 0.01, ^◆◆◆P < 0.001 for dishabituation. The symbol colors match with the appropriate genotype (grey for WT, magenta for Fmr1 KO). The statistical differences between genotypes were calculated using permutational non-paired t-test, *P < 0.05, **P < 0.01. For non-social odor discrimination (b, d) the comparison between first trials (T1) of two distinct odors (banana and vanilla) was evaluated using permutational paired t-test, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001. All data represent means ± SEM.