Fig. 3

Spontaneous social olfactory habituation/dishabituation and discrimination task in WT and Fmr1 KO mice. (a) Absolute (s) and relative (%) total sniffing duration during the social olfactory habituation/dishabituation test in WT and Fmr1 KO males (WT: n = 16, Fmr1 KO: n = 17). (b) Discrimination between two sex-group specific social odors (female and male urine, respectively) in WT and Fmr1 KO males. (c) Absolute (s) and relative (%) total sniffing duration during the social olfactory habituation/dishabituation test in WT and Fmr1 KO females (WT: n = 17, Fmr1 KO: n = 15). (d) Discrimination between two sex-group specific social odors (male and female urine, respectively) in WT and Fmr1 KO females. For the social olfactory habituation/dishabituation test (a, c) two sex-group specific odorants were used. The sex-group related odor-paired sessions were performed in following order: 1) opposite sex-group odor (SOC-O) and 2) same sex-group odor (SOC-S). Each session consists of three consecutive trials (T1–T3) with 2 min inter-session interval. The statistical significances of the habituation (T1 vs. T3) and dishabituation (T3 vs. T1 of following session) for each genotype were evaluated using permutational paired t-test, \(^{\blacktriangle \blacktriangle}\)P < 0.01, \(^{\blacktriangle \blacktriangle \blacktriangle}\)P < 0.001 for habituation, and ^◆P < 0.05, ^◆◆P < 0.01 for dishabituation. The symbol colors match with the appropriate genotype (grey for WT, magenta for Fmr1 KO). The statistical differences between genotypes were calculated using permutational non-paired t-test, *P < 0.05. For social odor discrimination (b, d) the comparison between first trials (T1) of SOC-O and SOC-S was evaluated using permutational paired t-test, ^###P < 0.001. All data represent means ± SEM.