Fig. 8
From: A new cancer/testis long noncoding RNA, the OTP-AS1 RNA
Two-round amplification of the studied transcript on the cDNA from different clinical tumor subjects. 1—lymphadenopathy of unknown origin (67), 2—non-Hodgkin’s lymphoma at stage II (82), 3—non-Hodgkin’s lymphoma at stage IV (113), 4—invasive ductal breast cancer at stage II (246), 6—negative control, first round of PCR, no template added, 7—negative control, second round of PCR, no template added, 8—positive control, first round of PCR with plasmid containing full transcript of newly identified gene 9—positive control, second round of PCR with plasmid containing full transcript of newly identified gene M1—GeneRuler™ 1 kb DNA ladder (Fermentas).
