Fig. 1 | Scientific Reports

Fig. 1

From: Publisher Correction: Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration

Fig. 1The alternative text for this image may have been generated using AI.

Novel laser model allows for accurate and reproducible optic nerve transections. A) Leica LMD7 laser microdissection experimental setup with Xla.Tg(tubb2b: mapt-GFP)Amaya transgenic X. laevis line. A’) Tadpoles are anesthetized and loaded onto small surgical beds made of 4% agarose inside a chamber slide, so they are flat with their dorsal side up. B) Pre-operative and post-operative views of the endogenously labelled optic nerve in the GFP channel. The gap in fluorescence in the post-operative image indicates the nerve was properly transected. C) Representative images of the precision and reproducibility of this model in 3 tadpoles with their nerves cut at the same distance from the eye. D) Longitudinal imaging of a tadpole after laser optic nerve transection. Axons distal to the injury site degenerate from day 0–2 post laser (PL), followed be several axons sprouting from the proximal cut end in all directions from the injury site by day 3 PL. Some regrowing axons quickly reach the brain after which this connection becomes reinforced by additional axons that follow the path of those pioneer axons. After 14 days, the optic nerve is virtually indistinguishable from the naïve (uninjured) optic nerve morphology.

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