Fig. 2 | Scientific Reports

Fig. 2

From: Publisher Correction: Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration

Fig. 2The alternative text for this image may have been generated using AI.

Pre-existing RGCs do not die and new RGCs are not generated from retinal progenitors after optic nerve transection. A) Control and experimental retinas at 1, 2, 3, 6, and 10 days post laser injury. EDU labeled cells are in magenta, cell nuclei are labelled in blue (DAPI). Tadpoles swam for 4 hours (pulse) in EdU-containing tadpole buffer (1X MMR), after which the retinas were collected for analysis of proliferating cells. B) Representative image of RGC layer in the retina labelled in cyan (RBPMS), Endogenous GFP (Tubb3), EDU in magenta and DAPI in blue. B’) Higher magnification image of cells in the ciliary marginal zone in the retina. C) 3 day cumulative EDU labelling of cells. Tadpoles swam from day 0–3, 3–7, or 7–10 PL. D) TUNEL positive control. TUNEL positive cells are in red, cell nuclei are labelled in blue (DAPI). DNA strand breaks were induced with DNase I. D’) Higher magnification image showing almost every cell nucleus is positive for TUNEL staining. E) TUNEL control and experimental retinas at 1, 2, 3, 6, and 10 days post laser injury. Experimental and control retinas do not differ from each other.

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