Fig. 1 | Scientific Reports

Fig. 1

From: Double step screening using endogenous marker improves relative gene targeting efficiency in Arabidopsis

Fig. 1

Simultaneous co-editing surrogate screening system. (a) A schematic representation of the simultaneous co-editing process for the purpose of efficient screening. The two sgRNAs in the construct target the gene of interest and the MAR1 locus. In each construct, the two sgRNAs can simultaneously generate DSBs at two distinct loci. (b) Overview of the co-editing surrogate double-step screening system. T1 transgenic plants were germinated directly on soil and subsequently screened by spraying with Basta and kanamycin.

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