Fig. 2
Statistical analysis of mutation rates by double-step screening. The mutation rates at endogenous target loci were analyzed. The mutation rates were analyzed in randomly selected Basta-resistant and kanamycin-resistant T1 transgenic plants, respectively. The numbers in parentheses indicate the number of randomly selected T1 plants. Pearson correlation coefficients (R) and the coefficient of determination (R2) for mutation rates between the target gene and the MAR1 locus were determined for the following: The following figures present the mutation rates of the SOS1 (a, b), TOR (c, d), DME N-terminal (e, f), and DME C-terminal (g, h) genes, respectively. The mutation rates of Basta-resistant (a, c, e, g) and kanamycin-resistant (b, d, f, h) T1 plants are presented. Two biological replicate experiments were conducted for TOR and the N- and C-termini of DME. i, Statistical analysis of mutation rates in target genes. The mutation rates of target genes were calculated following Basta (on the left) and kanamycin (on the right) screening. The standard deviation of the Student’s t-test was calculated (*P < 0.05, ***P < 0.001, ****P < 0.0001).
