Table 1 Precise GT efficiencies
Construct | Experiment | Basta screening | GT positive (biallelic) | GT efficiency (%) | kanamycin screening | GT positive (biallelic) | GT efficiency (%) |
|---|---|---|---|---|---|---|---|
SOS1-GFP-S | #1 | 96 | 1 | 1.04 | 22 | 1 | 4.55 |
#2 | 171 | 7 | 4.09 | 42 | 2 | 4.76 | |
SOS1-GFP-AS | #3 | 112 | 4 | 3.57 | 28 | 3 | 10.71 |
#4 | 237 | 12 (1) | 5.06 | 52 | 8 (1) | 15.38 | |
ROS1-FLAG | #5 | 185 | 7 | 3.78 | 50 | 3 | 6.00 |
#6 | 188 | 7 | 3.72 | 46 | 2 | 4.35 | |
NIA2-LUC | #7 | 168 | 1 | 0.59 | 49 | 1 | 2.04 |
#8 | 176 | 6 | 3.41 | 24 | 3 | 12.50 | |
Flag-DME | #9 | 142 | 2 | 1.41 | 30 | 0 | 0 |
