Fig. 1

Co-translational decay in C. albicans enables the study of codon-specific ribosome pauses. (A) Barplot of 5Pseq read distribution across all translation frames genome-wide showing F0 is the preferred translating frame in C. albicans, whereas S. cerevisae preferentially uses F1. (B). Metagene of 5’P signal over 6031 genes in C. albicans with an accumulation of reads -18 nucleotides of the termination site (with a shoulder at -17) representing a ribosome protection 1 bp larger than the accumulated reads at -17 nt that predominate in the 6601 5’P signals in S. cerevisae. (C) Metagene plot of 5Pseq read counts normalized as Counts per Million (CPM) shows a ribosome stall 14 nt upstream of translation start upon treatment with cycloheximide. (D,E). Arginine and proline depletion show ribosome stalls at proline (position -12 from the codon) and arginine (position -12 and -18 from the codon). (F, G) A scatter plot of the 5PSeq read counts at stalled positions -18 and -12 of ARG, PRO depleted (y-axis) compared to control (x-axis) show stalls are specific to the depleted amino acids experimentally.