Fig. 1
Expression constructs generated and compared in this research. pJL-TRBO = The empty pJL-TRBO vector backbone (blue)12 in which the expression constructs (green) were cloned into using PacI and AvrII restriction enzymes (black scissors). pRC-eGFP = Construct containing (5’ to 3’) the CaMV 35 S promoter, a synthetic 5’ UTR9, eGFP coding sequence, the CPMV 3’ UTR9, N. benthamiana Extensin and N. tabacum Actin-3 double terminator10, and RB7 matrix attachment region10 in the replicating vector pJL-TRBO (blue)12. pJL-TRBO-eGFP = Isolated eGFP coding sequence in the replicating vector pJL-TRBO. pRU-eGFP = Double UTR construct containing (5’ to 3’) the synthetic 5’ UTR, eGFP coding sequence, and CPMV 3’ UTR in the replicating vector pJL-TRBO. pR5-eGFP = 5’ UTR construct containing (5’ to 3’) the synthetic 5’ UTR and eGFP coding sequence in the replicating vector pJL-TRBO. pR3-eGFP = 3’ UTR construct containing (5’ to 3’) the eGFP coding sequence and CPMV 3’ UTR in the replicating vector pJL-TRBO.
