Fig. 5

Quantification of extracted protein between constructs. A – Raw western blot image showing total protein extracts of plants transformed with empty pJL-TRBO vector, pRC-eGFP, pJL-TRBO-eGFP and pR5-eGFP. Lane M shows a BioRad PrecisionPlus dual colour marker. The eGFP can be seen at 27 kDa. B – Histogram showing quantification of the 27 kDa eGFP bands relative to RbcL expression (Figure S3). pRC-eGFP (p < 0.0001) and pR5-eGFP (p < 0.001) have significantly higher fluorescence than the empty vector control. Both pRC-eGFP and pR5-eGFP show significantly higher fluorescence than the parent pJL-TRBO-eGFP vector (p < 0.001). The difference in expression between pRC-eGFP and pR5-eGFP was not statistically significant. Individual data points are shown. Significance values are: ns = not significant, *** = p < 0.001, **** = p < 0.0001.