Fig. 1
Plasmid DNA standards to establish standard melting curves for polymorphic sites in four genes. (a–d) The polymorphic site melting curves graph of CYP2C9*2 (rs1799853), CYP2C9*3 (rs1057910), VKORC1 (rs9923231) and CYP4F2*3 (rs2108622), respectively. As CYP2C9*2 gene for example, a single melting peak appeared and the Tm value was 49.99 ± 1.5 °C, the gene was judged to be wild-type. a single melting peak appeared and the Tm value was 59.08 ± 1.5 °C, the gene was judged to be homozygous variant. two melting peaks appeared, and the Tm values were 49.99 ± 1.5 °C and 59.08 ± 1.5 °C, respectively, the gene was judged to be a heterozygous variant. Genotyping of four polymorphic sites was achieved by the number of melting peaks and Tm values.
