Table 1 Gene polymorphism site primers, probe sequences.
Gene polymorphism site | Name (primers, probes) | Sequences (5’-3’) |
|---|---|---|
CYP2C9*2 (c.430 C > T, rs1799853) | F1 | 5’-GGGGAGGATGGAAAACAGAGA-3’ |
R1 | 5’-AGTAAGGTCAGTGATATGGAGTAGGG-3’ | |
P1 | 5’-FAM-TCCTCTTGAACACAGTCCT-BHQ1-3’ | |
CYP2C9*3 (c.1075 A > C, rs1057910) | F2 | 5’-TGTGCCATTTTTCTCCTTTTCC-3’ |
R2 | 5’-GATACTATGAATTTGGGGACTTCGA-3’ | |
P2 | 5’-HEX-AGGTCCAGAGATACCTTGAC-BHQ2-3’ | |
VKORC1 (c.-1639G > A, rs9923231) | F3 | 5’-CTGGGAAGTCAAGCAAGAGAAGA − 3’ |
R3 | 5’-CCAGGGTTCAAGTGGTTCTCG-3’ | |
P3 | 5’-ROX-ATTGGCCAGGTGCGGT-BHQ2-3’ | |
CYP4F2 (c.1297G > A, rs2108622) | F4 | 5’-CCTTCTCCTGACTGCTCCCTT-3’ |
R4 | 5’-CAGCCTTGGAGAGACAGACAGTT-3’ | |
P4 | 5’-Cy5-ACAACCCAGCTATGTG-BHQ2-3’ |
