Fig. 6 | Scientific Reports

Fig. 6

From: Multispectroscopic and computational insights into amyloid fibril formation of alpha lactalbumin induced by sodium hexametaphosphate

Fig. 6

Congo Red (CR) binding was performed to detect the amyloid fibrils in solution. The absorbance spectra of α-LA (0.2 mg mL−1) were obtained under different conditions, such as CR binding at pH 7.0 () and pH 2.0 () in the presence of 0.2 (), 0.3 (), and 0.5 () mM SHMP at pH 2.0. A fixed amount of CR (10 µM) was added to both the aggregated and nonaggregated samples and incubated for 30 min in the dark prior to the measurements.

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