Fig. 2

Construction of co-expression network for all differentially expressed genes. (A) Determination of soft threshold power for constructing a scale-free network using WGCNA. The plot shows the scale-free topology fit index (y-axis) across different soft threshold powers (x-axis). (B) Clustering dendrogram of gene modules built by WGCNA. Genes are grouped into modules based on their expression patterns. The branches of the dendrogram represent different modules, and each color indicates a distinct module. (C) Heatmap showing the correlation between module–trait of PCOS/control groups. Each row represents a module, and each column represents a trait (PCOS or control).