Fig. 5

Mitochondria in dnm1Δ cells are longer and less circular than mitochondria in WT cells. a) Wide-field fluorescence microscopy. The mitochondria were labeled with aconitase-GFP and the fluorescence images represent MIP (maximum intensity projection) images of z-stacks after deconvolution. MitoS-yeast_CB represents segmentation by the deep learning model MitoS trained to detect mitochondria from noisy background. DIC, differential interference contrast. Scale bar: 5 μm. b) Fragment length and c) circularity are presented as box plots with Tukey whiskers, means (+), medians (lines), and circles as outliers. The y-axis for fragment length is on the log2 scale. More than 150 cells from two different experiments were analyzed in each sample and the Mann–Whitney U test was used to test the difference between WT and dnm1Δ mitochondria.