Fig. 2 | Scientific Reports

Fig. 2

From: Goreisan promotes diuresis by regulating the abundance of aquaporin 2 phosphorylated at serine 269 through calcium-sensing receptor activation

Fig. 2

Effect of GRS on the phosphorylation of AQP2 at Ser269 in mouse kidneys. GRS (1–3 g/kg) or DW was orally administered to mice. Thirty minutes after oral administration, DDAVP (30 ng/kg) or saline was injected intraperitoneally at 90 mL/kg. Thirty minutes after the intraperitoneal injection, mice were perfusion-fixed with 4% paraformaldehyde, and kidneys were harvested. (a) Immunofluorescence staining images of phospho-AQP2 (pAQP2 [Ser269], white) and DAPI (blue) in renal tissue sections are presented. Scale bars, 1000 μm. (b) The ratio of the area of pAQP2 in kidney sections is presented as the mean ± SEM (n = 6). Differences between groups were evaluated using one-way ANOVA followed by a post-hoc Tukey’s multiple comparison test (**P < 0.01).

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