Fig. 4

Metabolic activity of the seeded on the intestinal cell lines cells (Caco-2 and LS174T monocultures, and co-cultured) over time on 5 (blue circle), 10 (red circle) and 15 (green circle)w/v% GelMA disks and control (voilet circle) (depicted with dot plots (average ± standard deviation), x-axis shows days after seeding, y-axis shows the absolute measured fluorescence of resorufin, metabolic activity was normalized according to surface area). The metabolic activity is shown until day 20 for Caco-2 cells, and until day 21 for the LS174T monoculture and Caco-2:LS174T co-culture. The metabolic activity of Caco-2 cells on the disks was measured after the dates depicted here and can be found in Figure S5. Next to the metabolic activity plots, confocal images of the cellular morphology of intestinal cells on GelMA disks at the end of the experiment for the mono- and co-cultures, per w/v % of GelMA (Red = Phalloidin-rhodamine staining for exoskeleton; Blue = DAPI staining for cell nuclei). For co-culture and the LS174T monocultures, the experiments were stopped after 24 days since cell seeding. For the Caco-2 cells, the experiment was stopped 34 days after seeding. The confocal images represent the state of the cell right at the point where the experiment was stopped and the cells were fixated, permeabilized and stained.