Fig. 3 | Scientific Reports

Fig. 3

From: Targeting cholangiocarcinoma cells by cold piezoelectric plasmas: in vitro efficacy and cellular mechanisms

Fig. 3

Spatiotemporally dependence of CPP effects on the CCA cell layer distribution. (a) 3D-view of the setup when the cell layer is exposed to a CPP device. In this scheme, the helium flow driver is absent to show plasma-liquid interaction inside a well. The bottom of the well is 2D-represented to focus on the cell nucleus observed from DAPI fluorescence images acquired along a radius of the well. Cell distribution observed on a radius of the well immediately (0 h) after CPP exposure, either for (b) HuCCT-1 cells or (c) EGI-1 cells. The same conditions are then observed 24 h after treatment and quantified for the two cell lines: (d) HuCCT-1 and (e) EGI-1. The nozzle radius is notified by gray background, respectively 5 mm for Pz-DBD and 0.5 mm for Pz-CD. Cell number is plotted in log-scale.

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