Table 1 Primary antibodies used for immunodetection and probe.
Name | Species | Clone | Reference | Manufacturer | Dilution |
|---|---|---|---|---|---|
GAPDH | M | 6C5 | sc-32233 | Santa Cruz Biotechnology | 1:5000 (WB) |
γH2AX | R | 20E3 | 9718 | CST | 1:1000 (WB), 1:200 (IF) |
pCHK1 | R | 133D3 | 2348 | CST | 1:1000 (WB) |
CHK1 | R | 2G1D5 | 2360 | CST | 1:1000 (WB) |
pp53 | R | / | 9284 | CST | 1:1000 (WB) |
p53 | M | DO-1 | sc-126 | Santa Cruz Biotechnology | 1:1000 (WB) |
Cleaved caspase 3 | R | 5A1E | 9664 | CST | 1:1000 (WB) |
Cleaved PARP | R | D64E10 | 5625 | CST | 1:300 (WB) |
Phalloidin-iFluor 488 | R | / | ab176753 | Abcam | 1:1000 (IF) |
