Table 3 Table illustrating the physicochemical and immunological behaviours of the designed vaccine.

Number of amino acids

427

Molecular weight

45,759.70

Formula

C₂₀₃₂H₃₁₈₄N₅₃₈O₆₃₅S₁₄

Theoretical isoelectric point (p^I)

5.59

Total number of atoms

6403

Aliphatic index

74.54

Instability index

21.37

Extinction coefficients (all pairs of Cys residues form cystines)

55,725

Extinction coefficients (all Cys residues are reduced)

55,350

Total number of negatively charged residues (Asp + Glu)

54

Total number of positively charged residues (Arg + Lys)

45

Estimated half-life

30 h (mammalian reticulocytes, in vitro)

 > 20 h (yeast, in vivo)

 > 10 h (E. coli, in vivo)

GRAVY

−0.219

Predicted scaled solubility

0.496

Antigenicity

0.946348/ Antigen

ANTIGENpro

VaxiJen v2.0

0.5104/ Probable antigen

Allergenicity

(AllerTOP v.2.0 & AllergenFP v.1.0)

Probable non-allergen

Toxicity (ToxinPred)

Non-toxic