Fig. 5
Benzethonium chloride enhances the ability of gefitinib to inhibit cell migration and invasion in NSCLC. (A, D) Migration maps were obtained via microscopy after cotreatment of H1975 cells with 10 μM gefitinib and benzethonium chloride (0, 2, 5, or 10 μM) for 24 h. The results were analyzed via ImageJ. (B, E) The images of invasive cells were obtained via microscopic photography after cotreatment of H1975 cells with 10 μM gefitinib and benzethonium chloride (0, 2, 5, or 10 μM) for 24 h. The results were analyzed via ImageJ. (C, F) Gefitinib at 10 μM and benzethonium chloride (0, 2, 5, or 10 μM) were administered to H1975 cells, and the width of the cell gap was measured by imaging at 0 h and 24 h, respectively. (G) Expression levels of EMT-related proteins after treatment of H1975 cells with different concentrations of benzethonium chloride (0, 2, 5, or 10 μM) and 10 μM gefitinib for 24 h. GAPDH was used as an internal reference.
