Fig. 8
From: PARP inhibition radiosensitizes BRCA1 wildtype and mutated breast cancer to proton therapy
Irradiation setup and physical characteristic of the photon and proton beams used for in vivo irradiations. (A) Restrainer used to immobilize the left leg. (B,C) Custom-designed jig in a water tank. The jig holds up to six mice, with their left legs (and tumors) inside a water tank. A half-beam block is used to spare the mouse’s body and internal organs from radiation. This setup allows each individual tumor to be precisely aligned to the radiation field. (D) Dose profile in the water tank used for photon (6 MV x-rays) irradiations with opposite lateral beams. (E) Dose profile and corresponding dose-weighted LET (LETD) profile in the water tank used for proton irradiations with lateral opposed beams. The proton dose profile and LETD was calculated with a validated Monte Carlo model of the beam line. Dashed lines represent the depth in which the tumors were exposed.
