Fig. 2 | Scientific Reports

Fig. 2

From: CpG hypomethylation at proximal promoter and 5’UTR along with IL6 signaling loop associates with MYD88 upregulation in epithelial ovarian cancer

Fig. 2

Variation of methylation of CpG sites in the MYD88 proximal promoter/5’UTR in EOC cells (a) Frequency spectrum of CpG sites of MYD88 proximal promoter/5’UTR in A2780 and OVCAR3 EOC cells. (b) The frequency profile of 27 methylated CpG sites in the MYD88 PPR/5’UTR (-244 ~ + 167 bp). Median CpG methylation frequency of 27 CpG sites in the MYD88 proximal promoter/5’UTR in A2780 EOC cells was significantly higher than that in OVAR3 cells (P < 0.01). (c) Median methylation frequency of 12 CpG sites in the MYD88 proximal promoter (-244~ -1 bp) in A2780 EOC cells was significantly higher than that in OVAR3 cells (P < 0.01). (d) Median methylation frequency of 15 CpG sites in the MYD88 5’UTR (+ 1 ~ + 167 bp) in A2780 EOC cells was significantly higher than that in OVAR3 cells (P < 0.01). (e) Median methylation frequency of CpG site located in GC box in the MYD88 proximal promoter (-36 ~ -31 bp) in A2780 EOC cells was significantly higher than that in OVAR3 cells (P < 0.01). (f) Median methylation frequency of one CpG site for SP1 binding motif (+ 108 ~ + 113 bp) and two CpG sites for IRF1 binding motif (+ 116 ~ + 133 bp) in the MYD88 5’UTR in A2780 EOC cells far exceeds that in OVAR3 cells (P < 0.01).

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