Fig. 4 | Scientific Reports

Fig. 4

From: CpG hypomethylation at proximal promoter and 5’UTR along with IL6 signaling loop associates with MYD88 upregulation in epithelial ovarian cancer

Fig. 4

Effect of IL-6R McAb blockade on SP1, IRF1, and MYD88 expression in EOC cells (a–c) IL-6R McAb significantly down-regulated the mRNA expression of SP1, IRF1 and MYD88 by m5’CpGlow OVCAR3 EOC cells in a time and concentration-dependent manner. (d–f) IL-6R McAb did not significantly impact on the mRNA expression of SP1, IRF1 and MYD88 by m5’CpGhigh A2780 EOC cells. (g) Diagram of western blot to analyze the relative protein expression of MYD88, SP1 and IRF1 after treatment with IL-6R McAb. The grouping of blots was cropped from different parts of the same gel. Original images of blots are presented in Supplementary Fig. 2. (h,i) Treatment with IL-6R McAb and treatment with IL-6R McAb significantly reduced protein expression of SP1, IRF1 and MYD88 in both OVCAR3 and A2780 EOC cells in a concentration-dependent manner (P < 0.01). Data are presented as mean ± SD (n = 3 each) statistical test was the unpaired t-test. **P < 0.01. (j) The depiction of a potential mechanism for IL-6-driven SP1/IRF1 signaling loop feedback upregulation of MYD88.

Back to article page