Fig. 6 | Scientific Reports

Fig. 6

From: CpG hypomethylation at proximal promoter and 5’UTR along with IL6 signaling loop associates with MYD88 upregulation in epithelial ovarian cancer

Fig. 6

Sequence map of MYD88 proximal promoter and 5’UTR. (a) The analyzed CpG sites are located at -244 ~ -1 bp of proximal promoter and + 1 ~ 167 bp of 5’UTR a mature transcript preceding the initiation codon (ATG). (b) The procedures of BSP. DNA agarose gel electropherogram of genomic DNA and 2 amplicons of BSP-DNA from normal ovarian tissues (lane 1 and 2), EOC tissues (lane 3 and 4) and A2780 EOC line (lane 5) and OVCAR3 EOC line (lane 6). (c), Cloning BSP-DNA PCR amplicons into TA cloning pEASY-T3 vector and transforming in competent cells. (d) Twenty white positive bacteria clones for sequencing with Sanger sequencing.

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