Fig. 3
siRNA interference technology on CDT1 expression. (A) The basal protein expression of CDT1 in immortalized gastric cell lines (GES-1) and human gastric cancer cells (AGS and MKN-45). (B,C) Western blot analysis of CDT1 protein inhibited by si-CDT1. They employed β-actin as the internal control. The information is displayed as the average ± standard deviation of three separate tests. (D) Representative immunofluorescence imaging of CDT1 protein staining in AGS and GES-1 cells. The cells were stained with the anti-CDT1 antibody (green), and DAPI (blue)was used to identify the nuclei (40×). Fluorescence intensity of CDT1. The information is displayed as the average ± standard deviation of three separate tests. **P < 0.01. Full-length blots/gels are presented in Supplementary Fig. 2.
