Fig. 6
Knockdown of SLCO4A1-AS1 downregulates stemness markers in GSC. (A) Significant positive correlation between SLCO4A1-AS1 and stemness markers in GSE4536. (B) SLCO4A1-AS1 expression in GSC and matched DGC measured by qRT-PCR (n = 3). (C) CD15, CD133and GFAP expression in GSC and matched DGC measured by qRT-PCR (n = 3). (D, E) Expression of SOX2, OLIG2, NES, CD133 and GFAP after knockdown of SLCO4A1-AS1 in GBM#P3 (D) and GBM#BG5 (E) (n = 3). (F, G) Knockdown of SLCO4A1-AS1 reduced sphere forming ability (F) and tumor sphere expansion capacity (G) in GBM#P3 and GBM#BG5 (n = 3). Scale bar = 50 μm. Data are presented as the mean ± SD. NS, not statistically significant; * P < 0.05; ** P < 0.01; *** P < 0.001. Student’s t-test (B, C), 1-way ANOVA (D, E, G) and 2-way ANOVA (D, E) were used for data analysis.
