Fig. 3
From: Genetic factors regulating Plasmodium falciparum gametocytogenesis identified by phenotypic screens
Genetic factors associated with late-stage gametocytes identified in the piggyBac phenotypic screen. (A) Relative differentiation of each piggyBac mutant in the library was determined by ranking mutants from low to high. The genes in the bottom indicated in red are significant and inferred as gametocyte hypoproducers (Log2fold-change < −0.75 and adjusted p < 0.05). The top ranked genes indicated in blue are significant and inferred as linked to gametocyte hyperproducers (Log2fold-change > 0.75 and adjusted p < 0.05). Hits highlighted include genes that have been previously associated with sexual development. The entire piggyBac screen dataset is provided in Table S3. (B) The mean number of transcripts per kilobase per million (TPM) of all the genes linked to hypoproducer and hyperproducer phenotype mutants were determined from published transcriptome sequencing RNAseq data from Lopez-Barragan et al.22 (Table S4) (mean and maximum and minimum; *p < 0.01 **p < 0.001, ***p < 0.0001, ****p < 0.00001, ANOVA follow by Turkey’s test). (C) Functional enrichment of significant gene ontology (GO) terms for early gametocyte hypoproducer and hyperproducer piggyBac mutant’s vs all other mutants in the library. The entire GO-dataset is provided in Table S6.
