Fig. 4

Gametocyte Development of Individual piggyBac Mutant Clones. (A) Five piggyBac mutant clones highlighted in the ranked plots from the early and late-stage gametocyte screens from the Half-k library (red for hypoproducer phenotype, blue for hyperproducer phenotype, and gray for neutral phenotype) were selected for phenotype validation. (B) Gametocyte development of these piggyBac mutants highlighted in (A) and NF54 were monitored over a period of 14 days. Gametocyte abundance was estimated by Giemsa-stained thin blood smears on Days 5, 8, 10 and 14 post-induction (Supplementary Table S9). To allow comparison among different lines, Gametocytemia (G %) for Days 5, 8, 10, and 14 were normalized by the parasitemia (P %) of Day 2 post-induction. At day 5, G% is commonly named gametocytes conversion rate (GCR%). The assay was performed with 3–5 biological replicates per parasite line (means with SD [error bars] are shown). Comparisons among parasite lines were analyzed using one-way ANOVA followed by Tukey’s test (*p < 0.01, **p < 0.001, ***p < 0.0001, ****p < 0.00001).