Fig. 3 | Scientific Reports

Fig. 3

From: Transduction of jellyfish superoxide dismutase mediated by TAT peptide ameliorates H2O2-induced oxidative stress in HaCaT cells

Fig. 3

Gel electrophoretic analysis of inducible expression of fusion protein TAT-CcSOD1. (A) TAT-CcSOD1 expression under different induction temperatures. SN, supernatant; SD, sediment. 0.2 mM IPTG was added into each bacterial solution for 6 h. (B) Ratio of TAT-CcSOD1 in soluble form was quantified using densitometric analysis by image J 1.8.0. (C) TAT-CcSOD1 expression under different IPTG concentrations. IPTG were added into each bacterial solution at 12 °C for 6 h, and the sonicated supernatant in each group was collected for gel electrophoresis analysis. (D) TAT-CcSOD1 expression under different induction time. 0.2 mM IPTG was added into each bacterial solution at 12 °C for 6, 12, 24 and 48 h. The sonicated sediment and supernatant in each group were collected for gel electrophoresis analysis. The quantification data were from at least three independent repetitions and the data were presented as means ± SD (n = 3) (ns stands for no significance, **P < 0.01).

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