Fig. 4 | Scientific Reports

Fig. 4

From: Transduction of jellyfish superoxide dismutase mediated by TAT peptide ameliorates H2O2-induced oxidative stress in HaCaT cells

Fig. 4

Purification and identification of fusion protein TAT-CcSOD1. (A) SDS-PAGE analysis of the samples obtained during protein purification. (B) Western blot analysis of the samples obtained during protein purification. Lane 1, supernatant of sonicated lysates after induction; lane 2, the flow through peak; lane 3–5, the eluted peaks under 15%, 30% and 50% elution buffer (20 mM NaH2PO4, 500 mM NaCl, 500 mM imidazole, pH 7.4), respectively. The position corresponding to TAT-CcSOD1 protein is indicated by red arrow.

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