Fig. 1 | Scientific Reports

Fig. 1

From: Expression of Yes-associated protein in endothelial cells of human corneas before and after storage in organ culture

Fig. 1

Morphological changes of the corneal endothelium during organ culture (OC). (A,B) Alizarin red staining of the human corneal endothelium. Corneal folds are indicated by black arrows. High magnification showing the morphology of corneal endothelial cells. (C) ZO-1 immunostaining of corneal endothelium (in red). Cell nuclei were counterstained with DAPI in blue. The white and yellow stars indicate pentagonal and heptagonal cells, respectively. The white arrows indicate the presence of ZO-1 condensates in the cytoplasm of endothelial cells (D) Evaluation of Endothelial Corneal Density (ECD) relative to OC storage duration. (E,F) Quantification of morphometric parameters obtained through automated image analysis, including the coefficient of variation and hexagonality expressed as a percentage. Statistical analysis was performed using the non-parametric Kruskal–Wallis test, with *p < 0.05 and **p < 0.01.

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