Fig. 7
Subcellular localization of phosphorylated YAP (p-YAP) at Serine 127 in relation to cell density and morphological alterations in human corneal endothelial cells cultured in vitro. Immunostaining of p-YAP (in green) and ZO-1 associated-Tight Junctions (TJ, in red) in the corneal endothelial cells (A) at different endothelial cell density and (B) with/without Ethylenediaminetetraacetic acid (EDTA)-induced disrupted tight junctions. Nuclei were counterstained by DAPI (in blue). The cells used were primary human corneal endothelial cells cultured from ex vivo corneas (n = 2 donors). The white arrows indicated a positive speckled antennae-like staining pattern for p-YAP Ser127 close to the nucleus in cell.
