Table 2 List of antibodies used to study corneal endothelial cells on flat-mounted whole corneas.
Target protein | Source | Isotype | Laboratory | Reference | Fixation | Secondary antibodies |
|---|---|---|---|---|---|---|
Anti-YAP1 (63.7) | Mouse | IgG2a | Santa Cruz Biotechnology, Inc. Germany | sc-101199 | Methanol | Goat anti-mouse IgG, Alexa Fluor™ 488, A32723, Invitrogen |
Anti-phospho-YAP1, p-YAP; D9W2I (ser127) | Rabbit | / | Cell signaling Technology, Danvers MA | 13008S | PFA | Goat anti-rabbit IgG, Alexa Fluor™ 488, A11034, Invitrogen |
Anti-ZO-1 | Rabbit | / | ThermoFisher Scientific Inc., USA | 40-2200 | Methanol PFA | Goat anti-rabbit IgG, Alexa Fluor™ 555, A21428, Invitrogen |
Mouse | IgG1 | BD Bioscience, Belgium | 610967 | PFA | 1. Goat anti-mouse IgG, Alexa Fluor™ 555, A11003, Invitrogen 2. Goat anti-mouse IgG1, Alexa Fluor™ 633, A21126, Invitrogen | |
Anti-acetylated α tubulin (6-11 B-1) | Mouse | IgG2b | Santa Cruz | sc-23950 | PFA | 1. Goat anti-mouse IgG, Alexa Fluor™ 555, A11003, Invitrogen 2. Goat anti-mouse IgG2b, Alexa Fluor™ 555, A21147, Invitrogen |
