Table 2 Accuracy and precision of the proposed HPLC method for the determination of AZT, FP and OXY in nasal mucosa.
Samples | AZT | FP | OXY | |||
|---|---|---|---|---|---|---|
Conc (µg/mL) | Recovery% | Conc (µg/mL) | Recovery% | Conc (µg/mL) | Recovery% | |
LLOQ | 8.00 | 103.95 | 10.00 | 103.67 | 10.00 | 103.66 |
LQC | 24.00 | 102.59 | 30.00 | 98.60 | 30.00 | 106.07 |
MQC | 62.50 | 101.83 | 50.00 | 99.23 | 62.50 | 99.89 |
HQC | 87.50 | 98.40 | 70.00 | 101.07 | 87.50 | 102.35 |
Mean ± SD | 101.69 ± 2.36 | 100.64 ± 2.28 | 102.99 ± 2.58 | |||
Samples | Intraday precision | |||||
|---|---|---|---|---|---|---|
AZT | FP | OXY | ||||
Conc (µg/mL) | Mean recovery*% ±RSD | Conc (µg/mL) | Mean recovery*% ±RSD | Conc (µg/mL) | Mean recovery*% ±RSD | |
LLOQ | 8.00 | 106.80 ± 3.83 | 10.00 | 107.67 ± 0.89 | 10.00 | 103.66 ± 8.34 |
LQC | 24.00 | 102.10 ± 0.46 | 30.00 | 98.05 ± 1.20 | 30.00 | 107.78 ± 5.98 |
MQC | 62.50 | 101.78 ± 0.09 | 50.00 | 98.88 ± 0.71 | 62.50 | 97.60 ± 5.52 |
HQC | 87.50 | 99.08 ± 0.98 | 70.00 | 100.75 ± 0.42 | 87.50 | 100.62 ± 2.02 |
Samples | Inter-day precision | |||||
|---|---|---|---|---|---|---|
AZT | FP | OXY | ||||
Conc (µg/mL) | Mean recovery*% ±RSD | Conc (µg/mL) | Mean recovery*% ±RSD | Conc (µg/mL) | Mean recovery*% ±RSD | |
LLOQ | 8.00 | 104.63 ± 5.57 | 10.00 | 106.30 ± 0.53 | 10.00 | 111.73 ± 7.63 |
LQC | 24.00 | 101.20 ± 1.41 | 30.00 | 102.76 ± 1.08 | 30.00 | 107.09 ± 9.86 |
MQC | 62.50 | 101.17 ± 0.07 | 50.00 | 98.96 ± 2.73 | 62.50 | 94.97 ± 6.25 |
HQC | 87.50 | 101.28 ± 0.18 | 70.00 | 99.67 ± 4.60 | 87.50 | 98.53 ± 3.71 |
