Fig. 1 | Scientific Reports

Fig. 1

From: Relationship among ultrasound features, pus Mycobacterium tuberculosis load, and efficacy of antituberculosis drugs in patients with necrotizing tuberculous lymphadenitis

Fig. 1

Bacterial load standard curve. The initial concentration of the bacterial solution was 2.1 × 105 CFU/L. A tenfold serial dilution was performed to obtain a range of bacterial concentrations. DNA was extracted from each diluted sample, and 2μL of the extracted DNA was used for real-time fluorescence PCR analysis. The experiment was repeated twice to calculate the average Ct value. By plotting the log10 (CFU) values on the x-axis and the average Ct values on the y-axis, a linear relationship was observed (y =  − 3.5299x + 36.326, R2 = 0.9817). This indicates a strong correlation between the Ct value and the logarithmic value of the bacterial load.

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